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KMID : 1001020030010030223
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Journal of Urologic Oncology
2003 Volume.1 No. 3 p.223 ~ p.228
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Cyclooxygenase-2 (COX-2) Expression in BBN-induced Rat Bladder Carcinogenesis
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Kim Jeong-Hyun
Kwak Cheol
Park In-Ae
Paick Jae-Seung
Lee Chong-Wook
Lee Eun-Sik
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Abstract
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Purpose: Cyclooxygenase-2(COX-2) is an early response gene induced by a variety of tumor promoters, cytokines, and growth factors. Much evidence suggests that COX-2 is increased in colon tumor tissues and is involved in colon cancer tumorigenesis. To determine the significance of COX-2 in tumorigenesis in the urinary bladder, the expression of COX-2 in transitional cell carcinoma and preneoplastic lesions of the bladder was examined.
Materials and Methods: Eight-week old Fisher 344 female rats were given 0.05% BBN diluted in drinking water for 8 weeks and sacrificed at 24 weeks of experiment. The urinary bladders were examined grossly and microscopically and routinely embedded in paraffin. Expression of COX-2 was analyzed immunohistochemically.
Results: Histopathologic findings of rat bladder cancer induced by BBN were very similar to human bladder cancer. Nineteen hyperplasia(H), 30 non-infiltrating tumors(compatible with human Ta tumor, NITCC) and 11 infiltrating tumors(compatible with human T1 tumors, ITCC) were found in experimental group and normal urothelium in controls. COX-2 was barely expressed in the normal epithelial cells, whereas it was expressed in 100% of H, 70% of NITCC and 45% of ITCC(p＜0.05).
Conclusions: Our study showed that in the early stage of bladder carcinogenesis COX-2 expression was increased. These results suggest that COX-2 might contribute to early bladder carcinogenesis.
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KEYWORD
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Bladder neoplasms, Carcinogenesis, Cyclooxygenase
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